Our Analytical Capabilities
We provide most commonly used proteomics workflows, from identification of a recombinant proteins resolved by SDS-PAGE to quantitative analyses of non-canonical PTMs, crosslinking mass spectrometry, and other complex experiments. The lab has experience with several proteolytic reagents, all major approaches for peptide quantification (including label-free quantification, TMT, and SILAC), on- and off- line chromatographic separations, protein crosslinking and in situ biotinylation, quantitative analysis of phosphorylation and other PTMs.
The facility relies on high resolution orbitrap mass spectrometers that enable both data-dependent, targeted (PRM, SIM), and data independent acquisition strategies, and is equipped with ETD fragmentation and ion mobility interfaces.
We also leverage several platforms for data analysis, depending on the specific experimental design.
Importantly, the MSSR is eager to develop and/or implement new workflows as needed to address specific biological questions.